Probing the Electrocatalytic Oxygen Reduction Reaction Reactivity of Immobilized Multicopper Oxidase CueO

Climent Paya, Victor Jose; Fu, Yongchun; Chumillas, Sara; Maestro, Beatriz; Li, Jianfeng; Kuzume, Akiyoshi; Keller, Stephan; Wandlowski, Thomas (2014). Probing the Electrocatalytic Oxygen Reduction Reaction Reactivity of Immobilized Multicopper Oxidase CueO. Journal of physical chemistry. C, 118(29), pp. 15754-15765. American Chemical Society 10.1021/jp5034382

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The bioelectrocatalytic (oxygen reduction reaction, ORR) properties of the multicopper oxidase CueO immobilized on gold electrodes were investigated. Macroscopic electrochemical techniques were combined with in situ scanning tunneling microscopy (STM) and surface-enhanced Raman spectroscopy at the ensemble and at the single-molecule level. Self-assembled monolayer of mercaptopropionic acid, cysteamine, and p-aminothiophenol were chosen as redox mediators. The highest ORR activity was observed for the protein attached to amino-terminated adlayers. In situ STM experiments revealed that the presence of oxygen causes distinct structure and electronic changes in the metallic centers of the enzyme, which determine the rate of intramolecular electron transfer and, consequently, affect the rate of electron tunneling through the protein. Complementary Raman spectroscopy experiments provided access for monitoring structural changes in the redox state of the type 1 copper center of the immobilized enzyme during the CueO-catalyzed oxygen reduction cycle. These results unequivocally demonstrate the existence of a direct electronic communication between the electrode substrate and the type 1 copper center.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Climent Paya, Victor Jose; Fu, Yongchun; Li, Jianfeng; Kuzume, Akiyoshi; Keller, Stephan and Wandlowski, Thomas

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
500 Science

ISSN:

1932-7447

Publisher:

American Chemical Society

Language:

English

Submitter:

Beatrice Niederhauser

Date Deposited:

26 Mar 2015 10:33

Last Modified:

25 Aug 2015 10:26

Publisher DOI:

10.1021/jp5034382

BORIS DOI:

10.7892/boris.65944

URI:

https://boris.unibe.ch/id/eprint/65944

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