Comparison of hepatic adaptation in extreme metabolic phenotypes observed in early lactation dairy cows on-farm

van Dorland, Hendrika Anette; Graber, Marco; Kohler, S.; Steiner, Adrian; Bruckmaier, Rupert (2014). Comparison of hepatic adaptation in extreme metabolic phenotypes observed in early lactation dairy cows on-farm. Journal of animal physiology and animal nutrition, 98(4), pp. 693-703. Blackwell Science 10.1111/jpn.12125

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The aim was to study the variation in metabolic responses in early-lactating dairy cows (n = 232) on-farm that were pre-selected for a high milk fat content (>45 g/l) and a high fat/protein ratio in milk (>1.5) in their previous lactation. Blood was assayed for concentrations of metabolites and hormones. Liver was measured for mRNA abundance of 25 candidate genes encoding enzymes and receptors involved in gluconeogenesis (6), fatty acid β-oxidation (6), fatty acid and triglyceride synthesis (5), cholesterol synthesis (4), ketogenesis (2) and the urea cycle (2). Two groups of cows were formed based on the plasma concentrations of glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyric acid (BHBA) (GRP+, high metabolic load; glucose <3.0 mm, NEFA >300 μm and BHBA >1.0 mm, n = 30; GRP-, low metabolic load; glucose >3.0 mm, NEFA <300 μm and BHBA <1.0 mm, n = 30). No differences were found between GRP+ and GRP- for the milk yield at 3 weeks post-partum, but milk fat content was higher (p < 0.01) for GRP+ than for GRP-. In week 8 post-partum, milk yield was higher in GRP+ in relation to GRP- (37.5 vs. 32.5 kg/d; p < 0.01). GRP+ in relation to GRP- had higher (p < 0.001) NEFA and BHBA and lower glucose, insulin, IGF-I, T3 , T4 concentrations (p < 0.01). The mRNA abundance of genes related to gluconeogenesis, fatty acid β-oxidation, fatty acid and triglyceride synthesis, cholesterol synthesis and the urea cycle was different in GRP+ compared to GRP- (p < 0.05), although gene transcripts related to ketogenesis were similar between GRP+ and GRP-. In conclusion, high metabolic load post-partum in dairy cows on-farm corresponds to differences in the liver in relation to dairy cows with low metabolic load, even though all cows were pre-selected for a high milk fat content and fat/protein ratio in milk in their previous lactation.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

van Dorland, Hendrika Anette; Graber, Marco; Steiner, Adrian and Bruckmaier, Rupert

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

0931-2439

Publisher:

Blackwell Science

Language:

English

Submitter:

Lorenzo Enrique Hernandez Castellano

Date Deposited:

07 Apr 2015 15:09

Last Modified:

12 Oct 2015 15:44

Publisher DOI:

10.1111/jpn.12125

PubMed ID:

24033645

Uncontrolled Keywords:

adaptation, dairy cow, liver, metabolic load

BORIS DOI:

10.7892/boris.66024

URI:

https://boris.unibe.ch/id/eprint/66024

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