Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks.

Schybli, Martina; Sigrist, Brigitte; Hess, Michael; van Leerdam, Bart; Hoop, Richard K; Vögtlin, Andrea (2014). Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks. Journal of veterinary diagnostic investigation, 26(2), pp. 189-194. Sage 10.1177/1040638714523426

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Between 2008 and 2012, commercial Swiss layer and layer breeder flocks experiencing problems in laying performance were sampled and tested for infection with Duck adenovirus A (DAdV-A; previously known as Egg drop syndrome 1976 virus). Organ samples from birds sent for necropsy as well as blood samples from living animals originating from the same flocks were analyzed. To detect virus-specific DNA, a newly developed quantitative real-time polymerase chain reaction method was applied, and the presence of antibodies against DAdV-A was tested using a commercially available enzyme-linked immunosorbent assay. In 5 out of 7 investigated flocks, viral DNA was detected in tissues. In addition, antibodies against DAdV-A were detected in all of the flocks.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Vögtlin, Andrea

Subjects:

600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

Sage

Language:

English

Submitter:

Barbara Gautschi-Steffen

Date Deposited:

09 Apr 2015 13:48

Last Modified:

15 Oct 2015 10:53

Publisher DOI:

10.1177/1040638714523426

PubMed ID:

24590667

Uncontrolled Keywords:

Duck adenovirus A, Egg drop syndrome 1976 virus, Switzerland, enzyme-linked immunosorbent assay, laying hens, real-time polymerase chain reaction assay

BORIS DOI:

10.7892/boris.66689

URI:

https://boris.unibe.ch/id/eprint/66689

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