Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Gottstein, Bruno; Eckert, J; Fey, Hans (1983). Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man. Zeitschrift für Parasitenkunde, 69(3), pp. 347-356. Springer 10.1007/BF00927876

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An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively. By affinity chromatography using rabbit anti hydatid fluid IgG coupled covalently to CNBr-Sepharose 4B a protein fraction (Em 1) containing shared antigens of both parasites could be isolated from an extract of E. multilocularis metacestode tissue. From the same source another antigen fraction (Em 2) with a high degree of specificity for E. multilocularis was prepared by immunosorption. Antigen Em 1 was equally sensitive for the detection of antibodies against E. granulosus and E. multilocularis, whereas antigen fraction Em 2 appeared to be more specific for E. multilocularis. A correct serological differential diagnosis was achieved in 95% of 57 confirmed cases of human cystic or alveolar echinococcosis by the simultaneous use of both antigen fractions in the ELISA and by comparison of their reactivities.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty

UniBE Contributor:

Gottstein, Bruno and Fey, Hans

Subjects:

600 Technology > 630 Agriculture
500 Science

ISSN:

0044-3255

Publisher:

Springer

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

14 Apr 2015 17:12

Last Modified:

18 Jul 2018 15:56

Publisher DOI:

10.1007/BF00927876

PubMed ID:

6192602

URI:

https://boris.unibe.ch/id/eprint/66737

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