An immunoassay for the detection of circulating antigens in human echinococcosis.

Gottstein, Bruno (1984). An immunoassay for the detection of circulating antigens in human echinococcosis. The American Journal of Tropical Medicine and Hygiene, 33(6), pp. 1185-1191. American Society of Tropical Medicine and Hygiene

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An immunoassay (double-antibody-sandwich-ELISA) was developed to detect circulating antigens (CAg) in patients with cystic (Echinococcus granulosus) echinococcosis. Echinococcus antigens derived from heterologous intermediate hosts were used to immunize rabbits and to purify the rabbit-IgG-fraction obtained by affinity-chromatography, thus avoiding major interference with host components. The purified rabbit anti-hydatid IgG was immunosorbed with bovine and human sera. One part of the resulting IgG served as coating agent in a double antibody sandwich-ELISA; the other part, coupled to alkaline phosphatase, as detecting conjugate. The specificity of the antibody reaction was demonstrated by immunoelectrophoresis. Sera of 21 patients with cystic echinococcosis were examined with this test system. In seven of the patients' sera CAg were detected in concentrations ranging between 310 ng and 680 ng protein per ml serum. Comparing pre- and postoperative serum samples obtained from nine patients operated on for cystic echinococcosis, four sera were found to be CAg-positive before and three after operation.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture

ISSN:

0002-9637

Publisher:

American Society of Tropical Medicine and Hygiene

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

14 Apr 2015 17:15

Last Modified:

18 Jul 2018 15:08

PubMed ID:

6391225

URI:

https://boris.unibe.ch/id/eprint/66738

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