The teleostean liver as an immunological organ: Intrahepatic immune cells (IHICs) in healthy and benzo[a]pyrene challenged rainbow trout (Oncorhynchus mykiss).

Möller, Anja-Maria; Korytář, Tomáš; Köllner, Bernd; Schmidt-Posthaus, Heike; Segner, Helmut (2014). The teleostean liver as an immunological organ: Intrahepatic immune cells (IHICs) in healthy and benzo[a]pyrene challenged rainbow trout (Oncorhynchus mykiss). Developmental and Comparative Immunology, 46(2), pp. 518-529. Elsevier 10.1016/j.dci.2014.03.020

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The existence of a resident population of intrahepatic immune cells (IHICs) is well documented for mammalian vertebrates, however, it is uncertain whether IHICs are present in the liver of teleostean fish. In the present study we investigated whether trout liver contains an IHIC population, and if so, what the relative cellular composition of this population is. The results provide clear evidence for the existence of an IHIC population in trout liver, which constitutes 15-29% of the non-hepatocytes in the liver, and with a cellular composition different to that of the blood leukocyte population. We also analyzed the response of IHICs to a non-infectious liver challenge with the hepatotoxic and immunotoxic chemical, benzo[a]pyrene (BaP). Juvenile trout were treated with BaP (25 or 100mg/kgbw) at levels sufficient to induce the molecular pathway of BaP metabolism while not causing pathological and inflammatory liver changes. The IHIC population responded to the BaP treatments in a way that differed from the responses of the leukocyte populations in trout blood and spleen, suggesting that IHICs are an independently regulated immune cell population.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Möller, Anja-Maria; Schmidt-Posthaus, Heike and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

0145-305X

Publisher:

Elsevier

Language:

English

Submitter:

Lucia Gugger-Raaflaub

Date Deposited:

24 Apr 2015 07:56

Last Modified:

12 Oct 2015 15:28

Publisher DOI:

10.1016/j.dci.2014.03.020

PubMed ID:

24718255

Uncontrolled Keywords:

Benzo[a]pyrene; Intrahepatic immune cells; Leukocytes; Liver; Rainbow trout

BORIS DOI:

10.7892/boris.67000

URI:

https://boris.unibe.ch/id/eprint/67000

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