Thyroid signaling in immune organs and cells of the teleost fish rainbow trout (Oncorhynchus mykiss).

Quesada Garcia, Maria del Alba; Valdehita, A; Kropf, Christian; Casanova-Nakayama, Ayako; Segner, Helmut; Navas, J M (2014). Thyroid signaling in immune organs and cells of the teleost fish rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 38(1), pp. 166-174. Elsevier Science 10.1016/j.fsi.2014.03.016

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Thyroid hormones are involved in modulating the immune system in mammals. In contrast, there is no information on the role played by these hormones in the immune system of teleost fish. Here we provide initial evidence for the presence of active thyroid signaling in immune organs and cells of teleosts. We demonstrate that immune organs (head kidney and spleen) and isolated leukocytes (from head kidney and peripheral blood) of the rainbow trout (Oncorhynchus mykiss) express both thyroid receptor α (THRA) and β (THRB). Absolute mRNA levels of THRA were significantly higher than those of THRB. THRA showed higher expression in immune organs and isolated immune cells compared to the reference organ, liver, while THRB showed the opposite. In vivo exposure of trout to triiodothryronine (T3) or the anti-thyroid agent propylthiouracil (PTU) altered THR expression in immune organs and cells. Effect of T3 and PTU over the relative expression of selected marker genes of immune cell subpopulations was also studied. Treatments changed the relative expression of markers of cytotoxic, helper and total T cells (cd4, cd8a, trb), B lymphocytes (mIgM) and macrophages (csf1r). These findings suggest that the immune system of rainbow trout is responsive to thyroid hormones.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Quesada Garcia, Maria del Alba; Kropf, Christian; Casanova, Ayako and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

1050-4648

Publisher:

Elsevier Science

Language:

English

Submitter:

Lucia Gugger-Raaflaub

Date Deposited:

24 Apr 2015 08:03

Last Modified:

12 Oct 2015 15:29

Publisher DOI:

10.1016/j.fsi.2014.03.016

PubMed ID:

24657316

Uncontrolled Keywords:

Immune cell markers; Propylthiouracil; Rainbow trout; Thyroid receptors; Triiodothryronine

BORIS DOI:

10.7892/boris.67009

URI:

https://boris.unibe.ch/id/eprint/67009

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