Bruno, Mark; Hofmann, Manuel; Vermathen, Martina; Alder, Adrian; Beyer, Peter; Al-Babili, Salim (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS letters, 588(9), pp. 1802-1807. Elsevier 10.1016/j.febslet.2014.03.041
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Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Vermathen, Martina |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
0014-5793 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Martina Vermathen |
Date Deposited: |
29 Apr 2015 15:25 |
Last Modified: |
05 Dec 2022 14:45 |
Publisher DOI: |
10.1016/j.febslet.2014.03.041 |
BORIS DOI: |
10.7892/boris.67526 |
URI: |
https://boris.unibe.ch/id/eprint/67526 |
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