On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

Bruno, Mark; Hofmann, Manuel; Vermathen, Martina; Alder, Adrian; Beyer, Peter; Al-Babili, Salim (2014). On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7. FEBS letters, 588(9), pp. 1802-1807. Elsevier 10.1016/j.febslet.2014.03.041

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Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Vermathen, Martina

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0014-5793

Publisher:

Elsevier

Language:

English

Submitter:

Martina Vermathen

Date Deposited:

29 Apr 2015 15:25

Last Modified:

02 Sep 2015 10:29

Publisher DOI:

10.1016/j.febslet.2014.03.041

BORIS DOI:

10.7892/boris.67526

URI:

https://boris.unibe.ch/id/eprint/67526

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