Barthel, Manja; Hapfelmeier, Siegfried; Quintanilla-Martínez, Leticia; Kremer, Marcus; Rohde, Manfred; Hogardt, Michael; Pfeffer, Klaus; Rüssmann, Holger; Hardt, Wolf-Dietrich (2003). Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host. Infection and immunity, 71(5), pp. 2839-2858. American Society for Microbiology 10.1128/IAI.71.5.2839-2858.2003
Full text not available from this repository. (Request a copy)Salmonella enterica subspecies 1 serovar Typhimurium is a principal cause of human enterocolitis. For unknown reasons, in mice serovar Typhimurium does not provoke intestinal inflammation but rather targets the gut-associated lymphatic tissues and causes a systemic typhoid-like infection. The lack of a suitable murine model has limited the analysis of the pathogenetic mechanisms of intestinal salmonellosis. We describe here how streptomycin-pretreated mice provide a mouse model for serovar Typhimurium colitis. Serovar Typhimurium colitis in streptomycin-pretreated mice resembles many aspects of the human infection, including epithelial ulceration, edema, induction of intercellular adhesion molecule 1, and massive infiltration of PMN/CD18(+) cells. This pathology is strongly dependent on protein translocation via the serovar Typhimurium SPI1 type III secretion system. Using a lymphotoxin beta-receptor knockout mouse strain that lacks all lymph nodes and organized gut-associated lymphatic tissues, we demonstrate that Peyer's patches and mesenteric lymph nodes are dispensable for the initiation of murine serovar Typhimurium colitis. Our results demonstrate that streptomycin-pretreated mice offer a unique infection model that allows for the first time to use mutants of both the pathogen and the host to study the molecular mechanisms of enteric salmonellosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research |
UniBE Contributor: |
Hapfelmeier, Siegfried Hektor |
ISSN: |
0019-9567 |
Publisher: |
American Society for Microbiology |
Language: |
English |
Submitter: |
Siegfried Hektor Hapfelmeier-Balmer |
Date Deposited: |
04 May 2015 14:19 |
Last Modified: |
05 Dec 2022 14:46 |
Publisher DOI: |
10.1128/IAI.71.5.2839-2858.2003 |
PubMed ID: |
12704158 |
Additional Information: |
Notes: PMC153285 |
URI: |
https://boris.unibe.ch/id/eprint/67838 |
Actions (login required)
 |
Edit item |