Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host.

Barthel, Manja; Hapfelmeier, Siegfried; Quintanilla-Martínez, Leticia; Kremer, Marcus; Rohde, Manfred; Hogardt, Michael; Pfeffer, Klaus; Rüssmann, Holger; Hardt, Wolf-Dietrich (2003). Pretreatment of mice with streptomycin provides a Salmonella enterica serovar Typhimurium colitis model that allows analysis of both pathogen and host. Infection and immunity, 71(5), pp. 2839-2858. American Society for Microbiology 10.1128/IAI.71.5.2839-2858.2003

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Salmonella enterica subspecies 1 serovar Typhimurium is a principal cause of human enterocolitis. For unknown reasons, in mice serovar Typhimurium does not provoke intestinal inflammation but rather targets the gut-associated lymphatic tissues and causes a systemic typhoid-like infection. The lack of a suitable murine model has limited the analysis of the pathogenetic mechanisms of intestinal salmonellosis. We describe here how streptomycin-pretreated mice provide a mouse model for serovar Typhimurium colitis. Serovar Typhimurium colitis in streptomycin-pretreated mice resembles many aspects of the human infection, including epithelial ulceration, edema, induction of intercellular adhesion molecule 1, and massive infiltration of PMN/CD18(+) cells. This pathology is strongly dependent on protein translocation via the serovar Typhimurium SPI1 type III secretion system. Using a lymphotoxin beta-receptor knockout mouse strain that lacks all lymph nodes and organized gut-associated lymphatic tissues, we demonstrate that Peyer's patches and mesenteric lymph nodes are dispensable for the initiation of murine serovar Typhimurium colitis. Our results demonstrate that streptomycin-pretreated mice offer a unique infection model that allows for the first time to use mutants of both the pathogen and the host to study the molecular mechanisms of enteric salmonellosis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research

UniBE Contributor:

Hapfelmeier, Siegfried Hektor

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Siegfried Hektor Hapfelmeier-Balmer

Date Deposited:

04 May 2015 14:19

Last Modified:

04 May 2015 15:10

Publisher DOI:

10.1128/IAI.71.5.2839-2858.2003

PubMed ID:

12704158

Additional Information:

Notes: PMC153285 Custom 1: papers3://publication/uuid/D40DE33C-AA70-4FAC-AAF2-5F50ADF26775 Date: 2003

URI:

https://boris.unibe.ch/id/eprint/67838

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