Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction.

Courts, Cornelius; Sauer, Eva; Hofmann, Yaiza; Madea, Burkhard; Schyma, Christian (2015). Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction. Journal of forensic sciences, 60(4), pp. 1016-1021. Wiley 10.1111/1556-4029.12746

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To date, there is no systematic investigation of the association of short tandem repeat (STR) typing success rate in soft tissues with different signs of putrefaction. Herein, putrefaction was rated using a newly developed 19-parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self-devised pentaplex PCR generating an "integrity score" (Si ). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si . The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp ) for each sample. Our results show that STR typing success rate can be predicted based on Sp before expensive multiplex STR profiling is performed.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Medicine

UniBE Contributor:

Schyma, Christian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-1198

Publisher:

Wiley

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

05 May 2015 16:53

Last Modified:

26 Jun 2016 02:02

Publisher DOI:

10.1111/1556-4029.12746

PubMed ID:

25808732

Uncontrolled Keywords:

DNA integrity, DNA profiling, disaster victim identification, forensic genetics, forensic science, putrefaction

BORIS DOI:

10.7892/boris.68066

URI:

https://boris.unibe.ch/id/eprint/68066

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