Neuroprotection through excitability and mTOR required in ALS motoneurons to delay disease and extend survival.

Saxena, Smita; Roselli, Francesco; Singh, Katyayani; Leptien, Kerstin; Julien, Jean-Pierre; Gros-Louis, Francois; Caroni, Pico (2013). Neuroprotection through excitability and mTOR required in ALS motoneurons to delay disease and extend survival. Neuron, 80(1), pp. 80-96. Cell Press 10.1016/j.neuron.2013.07.027

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Delaying clinical disease onset would greatly reduce neurodegenerative disease burden, but the mechanisms influencing early preclinical progression are poorly understood. Here, we show that in mouse models of familial motoneuron (MN) disease, SOD1 mutants specifically render vulnerable MNs dependent on endogenous neuroprotection signaling involving excitability and mammalian target of rapamycin (mTOR). The most vulnerable low-excitability FF MNs already exhibited evidence of pathology and endogenous neuroprotection recruitment early postnatally. Enhancing MN excitability promoted MN neuroprotection and reversed misfolded SOD1 (misfSOD1) accumulation and MN pathology, whereas reducing MN excitability augmented misfSOD1 accumulation and accelerated disease. Inhibiting metabotropic cholinergic signaling onto MNs reduced ER stress, but enhanced misfSOD1 accumulation and prevented mTOR activation in alpha-MNs. Modulating excitability and/or alpha-MN mTOR activity had comparable effects on the progression rates of motor dysfunction, denervation, and death. Therefore, excitability and mTOR are key endogenous neuroprotection mechanisms in motoneurons to counteract clinically important disease progression in ALS.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Saxena, Smita

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0896-6273

Publisher:

Cell Press

Language:

English

Submitter:

Prof. Smita Saxena

Date Deposited:

08 May 2015 11:31

Last Modified:

09 Sep 2015 10:23

Publisher DOI:

10.1016/j.neuron.2013.07.027

PubMed ID:

24094105

BORIS DOI:

10.7892/boris.68128

URI:

https://boris.unibe.ch/id/eprint/68128

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