Cholesterol acceptor capacity is preserved by different mechanisms in preterm and term fetuses.

Mohaupt, Markus (2014). Cholesterol acceptor capacity is preserved by different mechanisms in preterm and term fetuses. Biochimica et biophysica acta (BBA) - molecular and cell biology of lipids, 1841(2), pp. 251-258. Elsevier 10.1016/j.bbalip.2013.11.008

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Fetal serum cholesterol and lipoprotein concentrations differ between preterm and term born neonates. An imbalance of the flow of cholesterol from the sites of synthesis or efflux from cells of peripheral organs to the liver, the reverse cholesterol transport (RCT), is linked to atherosclerosis and cardiovascular disease (CVD). Preterm delivery is a risk factor for the development of CVD. Thus, we hypothesized that RCT is affected by a diminished cholesterol acceptor capacity in preterm as compared to term fetuses. Cholesterol efflux assays were performed in RAW264.7, HepG2, and HUVEC cell lines. In the presence and absence of ABC transporter overexpression by TO-901317, umbilical cord sera of preterm and term born neonates (n = 28 in both groups) were added. Lipid components including high density lipoprotein (HDL), low density lipoprotein (LDL), apolipoprotein A1, and apolipoprotein E were measured and related to fractional cholesterol efflux values. We found overall, fractional cholesterol efflux to remain constant between the study groups, and over gestational ages at delivery, respectively. However, correlation analysis revealed cholesterol efflux values to be predominantly related to HDL concentration at term, while in preterm neonates, cholesterol efflux was mainly associated with LDL In conclusion cholesterol acceptor capacity during fetal development is kept in a steady state with different mechanisms and lipid fractions involved at distinct stages during the second half of fetal development. However, RCT mechanisms in preterm neonates seem not to be involved in the development of CVD later in life suggesting rather changes in the lipoprotein pattern causative.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension

UniBE Contributor:

Mohaupt, Markus

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1388-1981

Publisher:

Elsevier

Language:

English

Submitter:

Geneviève Escher

Date Deposited:

12 May 2015 09:31

Last Modified:

02 Nov 2017 19:22

Publisher DOI:

10.1016/j.bbalip.2013.11.008

PubMed ID:

24291326

BORIS DOI:

10.7892/boris.68275

URI:

https://boris.unibe.ch/id/eprint/68275

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