Insulin, CCAAT/enhancer-binding proteins and lactate regulate the human 11β-hydroxysteroid dehydrogenase type 2 gene expression in colon cancer cell lines.

Andrieu, Thomas; Fustier, Pierre; Alikhani-Koupaei, Rasoul; Ignatova, Irena D; Guettinger, Andreas; Frey, Felix J; Frey, Brigitte M; Andrieu, Thomas; Fustier, Pierre; Alikhani, Rasoul; Ignatova, Irena Darieva; Güttinger, Andreas; Frey, Felix; Frey, Brigitte (2014). Insulin, CCAAT/enhancer-binding proteins and lactate regulate the human 11β-hydroxysteroid dehydrogenase type 2 gene expression in colon cancer cell lines. PLoS ONE, 9(8), e105354. Public Library of Science 10.1371/journal.pone.0105354

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11β-Hydroxysteroid dehydrogenases (11beta-HSD) modulate mineralocorticoid receptor transactivation by glucocorticoids and regulate access to the glucocorticoid receptor. The isozyme 11beta-HSD2 is selectively expressed in mineralocorticoid target tissues and its activity is reduced in various disease states with abnormal sodium retention and hypertension, including the apparent mineralocorticoid excess. As 50% of patients with essential hypertension are insulin resistant and hyperinsulinemic, we hypothesized that insulin downregulates the 11beta-HSD2 activity. In the present study we show that insulin reduced the 11beta-HSD2 activity in cancer colon cell lines (HCT116, SW620 and HT-29) at the transcriptional level, in a time and dose dependent manner. The downregulation was reversible and required new protein synthesis. Pathway analysis using mRNA profiling revealed that insulin treatment modified the expression of the transcription factor family C/EBPs (CCAAT/enhancer-binding proteins) but also of glycolysis related enzymes. Western blot and real time PCR confirmed an upregulation of C/EBP beta isoforms (LAP and LIP) with a more pronounced increase in the inhibitory isoform LIP. EMSA and reporter gene assays demonstrated the role of C/EBP beta isoforms in HSD11B2 gene expression regulation. In addition, secretion of lactate, a byproduct of glycolysis, was shown to mediate insulin-dependent HSD11B2 downregulation. In summary, we demonstrate that insulin downregulates HSD11B2 through increased LIP expression and augmented lactate secretion. Such mechanisms are of interest and potential significance for sodium reabsorption in the colon.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Nephrologie / Hypertonie
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Andrieu, Thomas; Fustier, Pierre; Alikhani, Rasoul; Ignatova, Irena Darieva; Güttinger, Andreas; Frey, Felix and Frey, Brigitte

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Funders:

[UNSPECIFIED] Swiss National Foundation for Scientific Research Nr. 3100-122135, 3100-61505.00, 3100A0-102153/1 and 3100A0-138670 (BMF & FJF
[UNSPECIFIED] National Centres of Competence in Research (NCCR)
[UNSPECIFIED] TransCure (FJF)

Language:

English

Submitter:

Brigitte Frey

Date Deposited:

17 Nov 2015 09:22

Last Modified:

17 Nov 2015 09:22

Publisher DOI:

10.1371/journal.pone.0105354

PubMed ID:

25133511

BORIS DOI:

10.7892/boris.68290

URI:

https://boris.unibe.ch/id/eprint/68290

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