In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter.

De Niz, Mariana; Helm, Susanne; Horstmann, Sebastian; Annoura, Takeshi; Del Portillo, Hernando A; Khan, Shahid M; Heussler, Volker (2015). In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter. PLoS ONE, 10(4), e0123473. Public Library of Science 10.1371/journal.pone.0123473

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Little is known about stage-specific gene regulation in Plasmodium parasites, in particular the liver stage of development. We have previously described in the Plasmodium berghei rodent model, a liver stage-specific (lisp2) gene promoter region, in vitro. Using a dual luminescence system, we now confirm the stage specificity of this promoter region also in vivo. Furthermore, by substitution and deletion analyses we have extended our in vitro characterization of important elements within the promoter region. Importantly, the dual luminescence system allows analyzing promoter constructs avoiding mouse-consuming cloning procedures of transgenic parasites. This makes extensive mutation and deletion studies a reasonable approach also in the malaria mouse model. Stage-specific expression constructs and parasite lines are extremely valuable tools for research on Plasmodium liver stage biology. Such reporter lines offer a promising opportunity for assessment of liver stage drugs, characterization of genetically attenuated parasites and liver stage-specific vaccines both in vivo and in vitro, and may be key for the generation of inducible systems.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

De Niz Hidalgo, Mariana and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Volker Heussler

Date Deposited:

01 May 2015 10:25

Last Modified:

16 Jan 2019 15:29

Publisher DOI:

10.1371/journal.pone.0123473

PubMed ID:

25874388

BORIS DOI:

10.7892/boris.68495

URI:

https://boris.unibe.ch/id/eprint/68495

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