Recent improvements in antigene technology

Buchini, S.; Leumann, Christian (2003). Recent improvements in antigene technology. Current opinion in chemical biology, 7(6), pp. 717-726. Elsevier 10.1016/j.cbpa.2003.10.007

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DNA triple helix based approaches to control and modulate cellular functions on the level of genomic DNA (antigene technology) suffered in the past from a stepmother like treatment in comparison to the flourishing field of oligonucleotide based control of translation (antisense technology). This was mostly due to lack of affinity of triplex forming oligonucleotides (TFOs) to their DNA target, to sequence restriciton constraints imposed by the triple helical recogniton motifs and by open questions to the accessibility of the target DNA. Recent developments in the area have brought about new bases that specifically recognize pyrimidine-purine inversion sites as well as sugar modifications, e.g. the 2'-aminoethoxy-oligonucleotides or oligonucleotides based on the locked nucleic acid (LNA) sugar unit, that greatly enhance triplex stability and alleviate in part the sequence restriction constraints. With this, sequence specific genomic DNA manipulation starts to become a useful tool in biotechnology

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Leumann, Christian

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1367-5931

Publisher:

Elsevier

Language:

English

Submitter:

Christian Leumann

Date Deposited:

19 May 2015 08:57

Last Modified:

19 May 2015 08:57

Publisher DOI:

10.1016/j.cbpa.2003.10.007

Uncontrolled Keywords:

ACID antigene Antisense base bases cellular DNA helix inversion LNA motif oligonucleotide Oligonucleotides restriction site stability sugar triple triple helix Triplex

BORIS DOI:

10.7892/boris.68569

URI:

https://boris.unibe.ch/id/eprint/68569

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