Propagation of melting cooperativity along the phosphodiester backbone of DNA

Becaud, J.; Pompizi, I.; Leumann, Christian (2003). Propagation of melting cooperativity along the phosphodiester backbone of DNA. Journal of the American Chemical Society, 125(50), pp. 15338-15342. American Chemical Society 10.1021/ja035313j

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The role of the DNA phosphodiester backbone in the transfer of melting cooperativity between two helical domains was experimentally addressed with a helix-bulge-helix DNA model, in which the bulge consisted of a varying number of either conformationally flexible propanediol or conformationally constrained bicyclic anucleosidic phosphodiester backbone units. We found that structural communication between two double helical domains is transferred along the DNA backbone over the equivalent of ca. 12-20 backbone units, depending on whether there is a symmetric or asymmetric distribution of the anucleosidic units on both strands. We observed that extension of anucleosidic units on one strand only suffices to disrupt cooperativity transfer in a similar way as if extension occurs on both strands, indicating that the length of the longest anucleosidic inset determines cooperativity transfer. Furthermore, conformational rigidity of the sugar unit increases the distance of coopertivity transfer along the phosphodiester backbone. This is especially the case when the units are asymmetrically distributed in both strands

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Leumann, Christian


500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry




American Chemical Society




Christian Leumann

Date Deposited:

19 May 2015 08:28

Last Modified:

05 Dec 2022 14:46

Publisher DOI:


PubMed ID:


Additional Information:

Notes: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, SwitzerlandFAU - Becaud, Jessica

Uncontrolled Keywords:

backbone chemistry distribution DNA double flexible Nucleic Acid Conformation nucleosides Oligonucleotides Phosphoric Acid Esters sugar Support,Non-U.S.Gov't Transition Temperature




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