DNA binding properties of oligodeoxynucleotides containing pyrrolidino C-nucleosides

Häberli, A.; Leumann, Christian (2002). DNA binding properties of oligodeoxynucleotides containing pyrrolidino C-nucleosides. Organic letters, 4(19), pp. 3275-3278. American Chemical Society 10.1021/ol026537u

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We have incorporated pyrrolidino-C-nucleosides (pyrrolidino-pseudonucleosides) containing the base uracil and N-1-methyl uracil into oligodeoxynucleotides and compared their thermal duplex and triplex stabilities with unmodified or pseudouridine-containing oligodeoxynucleotides. We find relative destabilizations of triplex formation by ca. -13 to -1 degrees C per modification (relative to thymidine) in a strongly sequence dependent mode. Duplex formation is less destabilizing and more homogeneous with -4 to -2 degrees C per modification

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Leumann, Christian

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1523-7060

Publisher:

American Chemical Society

Language:

English

Submitter:

Christian Leumann

Date Deposited:

19 May 2015 10:51

Last Modified:

19 May 2015 10:51

Publisher DOI:

10.1021/ol026537u

PubMed ID:

12227767

Uncontrolled Keywords:

base binding C-nucleosides DNA duplex DUPLEXES Oligodeoxynucleotides properties pyrrolidino-DNA stability thermal thymidine Triplex Uracil

BORIS DOI:

10.7892/boris.68582

URI:

https://boris.unibe.ch/id/eprint/68582

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