Groebke, Katrin; Leumann, Christian (1990). A Method for Preparing Oligodeoxynucleotides Containing an Apurinic Site. Helvetica chimica acta, 73(3), pp. 608-617. Verlag Helvetica Chimica Acta 10.1002/hlca.19900730309
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10.1002/hlca.19900730309.abs In three steps, 2-deoxy-D-ribose has been converted into a phosphoramidite building block bearing a (t-Bu)Me2Si protecting group at the OH function of the anomeric centre of the furanose ring. This building block was subsequently incorporated into DNA oligomers of various base sequences using the standard phosphoramidite protocol for automated DNA synthesis. The resulting silyl-oligomers have been purified by HPLC and selectively desilylated to the corresponding free apurinic DNA sequences. The hexamer d (A-A-A-A-X-A) (X representing the apurinic site) which was prepared in this way was characterized by 1H- and 31P-NMR spectroscopy. The other sequences as well as their fragments, which formed upon treatment with alkali base, were analyzed by polyacrylamide gel electrophoresis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Leumann, Christian |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
0018-019X |
Publisher: |
Verlag Helvetica Chimica Acta |
Language: |
English |
Submitter: |
Christian Leumann |
Date Deposited: |
21 May 2015 09:33 |
Last Modified: |
05 Dec 2022 14:47 |
Publisher DOI: |
10.1002/hlca.19900730309 |
BORIS DOI: |
10.7892/boris.68641 |
URI: |
https://boris.unibe.ch/id/eprint/68641 |
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