A Method for Preparing Oligodeoxynucleotides Containing an Apurinic Site

Groebke, Katrin; Leumann, Christian (1990). A Method for Preparing Oligodeoxynucleotides Containing an Apurinic Site. Helvetica chimica acta, 73(3), pp. 608-617. Verlag Helvetica Chimica Acta 10.1002/hlca.19900730309

[img] Text
19900730309_ftp.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (673kB) | Request a copy

10.1002/hlca.19900730309.abs In three steps, 2-deoxy-D-ribose has been converted into a phosphoramidite building block bearing a (t-Bu)Me2Si protecting group at the OH function of the anomeric centre of the furanose ring. This building block was subsequently incorporated into DNA oligomers of various base sequences using the standard phosphoramidite protocol for automated DNA synthesis. The resulting silyl-oligomers have been purified by HPLC and selectively desilylated to the corresponding free apurinic DNA sequences. The hexamer d (A-A-A-A-X-A) (X representing the apurinic site) which was prepared in this way was characterized by 1H- and 31P-NMR spectroscopy. The other sequences as well as their fragments, which formed upon treatment with alkali base, were analyzed by polyacrylamide gel electrophoresis.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Leumann, Christian

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0018-019X

Publisher:

Verlag Helvetica Chimica Acta

Language:

English

Submitter:

Christian Leumann

Date Deposited:

21 May 2015 09:33

Last Modified:

21 May 2015 09:33

Publisher DOI:

10.1002/hlca.19900730309

BORIS DOI:

10.7892/boris.68641

URI:

https://boris.unibe.ch/id/eprint/68641

Actions (login required)

Edit item Edit item
Provide Feedback