Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.

Askjaer, Peter; Galy, Vincent; Meister, Peter (2014). Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans. Methods in cell biology, 122, pp. 277-310. Academic Press 10.1016/B978-0-12-417160-2.00013-8

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The nematode Caenorhabditis elegans is characterized by many features that make it highly attractive to study nuclear pore complexes (NPCs) and nucleocytoplasmic transport. NPC composition and structure are highly conserved in nematodes and being amenable to a variety of genetic manipulations, key aspects of nuclear envelope dynamics can be observed in great details during breakdown, reassembly, and interphase. In this chapter, we provide an overview of some of the most relevant modern techniques that allow researchers unfamiliar with C. elegans to embark on studies of nucleoporins in an intact organism through its development from zygote to aging adult. We focus on methods relevant to generate loss-of-function phenotypes and their analysis by advanced microscopy. Extensive references to available reagents, such as mutants, transgenic strains, and antibodies are equally useful to scientists with or without prior C. elegans or nucleoporin experience.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Meister, Pierre

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0091-679X

Publisher:

Academic Press

Language:

English

Submitter:

Pierre Meister

Date Deposited:

05 May 2015 08:08

Last Modified:

05 May 2015 08:08

Publisher DOI:

10.1016/B978-0-12-417160-2.00013-8

PubMed ID:

24857735

Uncontrolled Keywords:

CRISPR-Cas9; Caenorhabditis elegans; Live imaging; Nuclear pore complex; Nucleocytoplasmic transport; Nucleoporin; RNAi; Super-resolution microscopy; npc; npp

BORIS DOI:

10.7892/boris.68768

URI:

https://boris.unibe.ch/id/eprint/68768

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