Modern techniques for the analysis of chromatin and nuclear organization in C. elegans.

Askjaer, Peter; Ercan, Sevinç; Meister, Peter (2014). Modern techniques for the analysis of chromatin and nuclear organization in C. elegans. WormBook : the online review of C. elegans biology, pp. 1-35. wormbook.org. 10.1895/wormbook.1.169.1

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In recent years, Caenorhabditis elegans has emerged as a new model to investigate the relationships between nuclear architecture, cellular differentiation, and organismal development. On one hand, C. elegans with its fixed lineage and transparent body is a great model organism to observe gene functions in vivo in specific cell types using microscopy. On the other hand, two different techniques have been applied in nematodes to identify binding sites for chromatin-associated proteins genome-wide: chromatin immunoprecipitation (ChIP), and Dam-mediated identification (DamID). We summarize here all three techniques together as they are complementary. We also highlight strengths and differences of the individual approaches.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Meister, Pierre

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1551-8507

Publisher:

wormbook.org.

Language:

English

Submitter:

Pierre Meister

Date Deposited:

05 May 2015 08:00

Last Modified:

05 May 2015 08:00

Publisher DOI:

10.1895/wormbook.1.169.1

PubMed ID:

24706462

BORIS DOI:

10.7892/boris.68769

URI:

https://boris.unibe.ch/id/eprint/68769

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