Phyllotaxis involves auxin drainage through leaf primordia

Deb, Yamini; Marti, Dominik; Frenz, Martin; Kuhlemeier, Cris; Reinhardt, Didier (2015). Phyllotaxis involves auxin drainage through leaf primordia. Development, 142(11), pp. 1992-2001. Company of Biologists Limited 10.1242/dev.121244

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The spatial arrangement of leaves and flowers around the stem, known as phyllotaxis, is controlled by an auxin-dependent reiterative mechanism that leads to regular spacing of the organs and thereby to remarkably precise phyllotactic patterns. The mechanism is based on the active cellular transport of the phytohormone auxin by cellular influx and efflux carriers, such as AUX1 and PIN1. Their important role in phyllotaxis is evident from mutant phenotypes, but their exact roles in space and time are difficult to address due to the strong pleiotropic phenotypes of most mutants in phyllotaxis. Models of phyllotaxis invoke the accumulation of auxin at leaf initials and removal of auxin through their developing vascular strand, the midvein. We have developed a precise microsurgical tool to ablate the midvein at high spatial and temporal resolution in order to test its function in leaf formation and phyllotaxis. Using amplified femtosecond laser pulses, we ablated the internal tissues in young leaf primordia of tomato (Solanum lycopersicum) without damaging the overlying L1 and L2 layers. Our results show that ablation of the future midvein leads to a transient accumulation of auxin in the primordia and to an increase in their width. Phyllotaxis was transiently affected after midvein ablations, but readjusted after two plastochrons. These results indicate that the developing midvein is involved in the basipetal transport of auxin through young primordia, which contributes to phyllotactic spacing and stability.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Applied Physics
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Deb, Yamini; Marti, Dominik; Frenz, Martin; Kuhlemeier, Cris and Reinhardt, Didier

Subjects:

600 Technology > 620 Engineering
500 Science > 580 Plants (Botany)

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

26 May 2015 11:45

Last Modified:

06 Jun 2017 15:39

Publisher DOI:

10.1242/dev.121244

Uncontrolled Keywords:

Phyllotaxis, Patterning, Meristem, Laser ablation, Auxin, PIN1, Tomato

BORIS DOI:

10.7892/boris.68867

URI:

https://boris.unibe.ch/id/eprint/68867

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