The impact of testosterone, tibolone and black cohosh on purified mammary and placental 17β-hydroxysteroid dehydrogenase type 1

Stute, Petra; Kalkhake, Kathrin; Kiesel, Ludwig; Götte, Martin (2015). The impact of testosterone, tibolone and black cohosh on purified mammary and placental 17β-hydroxysteroid dehydrogenase type 1. Journal of enzyme inhibition and medicinal chemistry, 30(3), pp. 448-457. Informa Healthcare 10.3109/14756366.2014.943205

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Abstract Context: Mammary and placental 17β-hydroxysteroid dehydrogenase type 1 (17βHSD1). Objective: To assess the impact of testosterone, tibolone, and black cohosh on purified mammary and placental 17βHSD1. Materials and methods: 17βHSD1 was purified from human mammary gland and placenta by column chromatography, its activity was monitored by a radioactive activity assay, and the degree of purification was determined by gel electrophoresis. Photometric cofactor transformation analysis was performed to assess 17βHSD1 activity without or in presence of testosterone, tibolone and black cohosh. Results: 17βHSD1 from both sources displayed a comparable basal activity. Testosterone and tibolone metabolites inhibited purified mammary and placental 17βHSD1 activity to a different extent, whereas black cohosh had no impact. Discussion: Studies on purified enzymes reveal the individual action of drugs on local regulatory mechanisms thus helping to develop more targeted therapeutic intervention. Conclusion: Testosterone, tibolone and black cohosh display a beneficial effect on local mammary estrogen metabolism by not affecting or decreasing local estradiol exposure.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

Stute, Petra

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1475-6374

Publisher:

Informa Healthcare

Language:

English

Submitter:

Monika Zehr

Date Deposited:

13 May 2015 11:00

Last Modified:

14 Jul 2015 01:30

Publisher DOI:

10.3109/14756366.2014.943205

PubMed ID:

25109879

Uncontrolled Keywords:

17βHSD1; Black cohosh; mammary gland; placenta; testosterone; tibolone

URI:

https://boris.unibe.ch/id/eprint/68932

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