Map positions of third chromosomal female sterile and lethal mutations of Drosophila melanogaster

Christopher, Schuetze; Peters, Michelle; Jia-Jen, Duong; Matthieu, Cavey; Dörig, Ruth; Lasko, Paul; Suter, Beat (2004). Map positions of third chromosomal female sterile and lethal mutations of Drosophila melanogaster. Genome, 47(5), pp. 832-838. National Research Council of Canada 10.1139/g04-052

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Chromosomal mutations induced by ethyl methanesulfonate (EMS) treatment can cause female sterility or maternal-effect lethality in Drosophila. EMS is particularly useful to researchers because it creates mutations independent of position effects. However, because researchers have little control over the chromosomal site of mutation, post-mutagenic genetic mapping is required to determine the cytological location of the mutation. To make a valuable set of mutants more useful to the research community, we have mapped the uncharacterized part of the female-sterile – maternal-effect lethal Tübingen collection. We mapped 49 female-sterile – maternal-effect lethal alleles and 72 lethal alleles to individual deficiency intervals on the third chromosome. In addition, we analyzed the phenotype of ovaries resulting from female sterile mutations. The observed phenotypes range from tumorous ovaries and early blocks in oogenesis, to later blocks, slow growth, blocks in stage 10, to apparently full development of the ovary. The mapping and phenotypic characterization of these 121 mutations provide the necessary information for the researcher to consider a specific mutant as a candidate for their gene of interest.Key words: Drosophila melanogaster, oogenesis, female sterile, maternal-effect lethal, EMS-induced mutations.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0831-2796

Publisher:

National Research Council of Canada

Language:

English

Submitter:

Beat Suter

Date Deposited:

26 May 2015 15:46

Last Modified:

26 May 2015 15:46

Publisher DOI:

10.1139/g04-052

PubMed ID:

15499397

URI:

https://boris.unibe.ch/id/eprint/68942

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