Characterization of the Drosophila protein arginine methyltransferases DART1 and DART4

BOULANGER, Marie-Chloé; MIRANDA, Tina Branscombe; Steven, CLARKE; DI FRUSCIO, Marco; Suter, Beat; LASKO, Paul; RICHARD, Stéphane (2004). Characterization of the Drosophila protein arginine methyltransferases DART1 and DART4. Biochemical journal, 379(Pt2), pp. 283-289. Portland Press 10.1042/BJ20031176

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The role of arginine methylation in Drosophila melanogaster is unknown. We identified a family of nine PRMTs (protein arginine methyltransferases) by sequence homology with mammalian arginine methyltransferases, which we have named DART1 to DART9 ( Drosophila arginine methyltransferases 1-9). In keeping with the mammalian PRMT nomenclature, DART1, DART4, DART5 and DART7 are the putative homologues of PRMT1, PRMT4, PRMT5 and PRMT7. Other DART family members have a closer resemblance to PRMT1, but do not have identifiable homologues. All nine genes are expressed in Drosophila at various developmental stages. DART1 and DART4 have arginine methyltransferase activity towards substrates, including histones and RNA-binding proteins. Amino acid analysis of the methylated arginine residues confirmed that both DART1 and DART4 catalyse the formation of asymmetrical dimethylated arginine residues and they are type I arginine methyltransferases. The presence of PRMTs in D. melanogaster suggest that flies are a suitable genetic system to study arginine methylation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0264-6021

Publisher:

Portland Press

Language:

English

Submitter:

Beat Suter

Date Deposited:

26 May 2015 16:00

Last Modified:

26 May 2015 16:00

Publisher DOI:

10.1042/BJ20031176

PubMed ID:

14705965

URI:

https://boris.unibe.ch/id/eprint/68944

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