Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study

Brolese, Eliane Lucia; Buser, Daniel; Kuchler, Ulrike; Schaller, Benoît; Gruber, Reinhard (2015). Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study. Clinical oral implants research, 26(10), pp. 1211-1214. Wiley-Blackwell 10.1111/clr.12432

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Abstract
OBJECTIVE:
Signaling molecules derived from osteocytes have been proposed as a mechanism by which autografts contribute to bone regeneration. However, there have been no studies that determined the role of osteocytes in bone grafts.
MATERIAL AND METHOD:
Herein, it was examined whether bone chips and demineralized bone matrix release sclerostin and FGF-23, both of which are highly expressed by osteocytes.
RESULTS:
Bone grafts from seven donors were placed in culture medium. Immunoassay showed that bone chips released sclerostin (median 1.0 ng/ml) and FGF-23 (median 9.8 relative units/ml) within the first day, with declining levels overtime. Demineralized bone matrix also released detectable amounts of sclerostin into culture medium, while FGF-23 remained close to the detection limit. In vitro expanded isolated bone cells failed to release detectable amounts of sclerostin and FGF-23.
CONCLUSION:
These results suggest that autografts but also demineralized bone matrix can release signaling molecules that are characteristically produced by osteocytes.
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
KEYWORDS:
FGF-23; autologous bone; bone grafts; demineralized bone matrix; osteocytes; sclerostin

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