Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study

Brolese, Eliane Lucia; Buser, Daniel; Kuchler, Ulrike; Schaller, Benoît; Gruber, Reinhard (2015). Human bone chips release of sclerostin and FGF-23 into the culture medium: an in vitro pilot study. Clinical oral implants research, 26(10), pp. 1211-1214. Wiley-Blackwell 10.1111/clr.12432

[img] Text
clr12432.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (185kB) | Request a copy

Abstract OBJECTIVE: Signaling molecules derived from osteocytes have been proposed as a mechanism by which autografts contribute to bone regeneration. However, there have been no studies that determined the role of osteocytes in bone grafts. MATERIAL AND METHOD: Herein, it was examined whether bone chips and demineralized bone matrix release sclerostin and FGF-23, both of which are highly expressed by osteocytes. RESULTS: Bone grafts from seven donors were placed in culture medium. Immunoassay showed that bone chips released sclerostin (median 1.0 ng/ml) and FGF-23 (median 9.8 relative units/ml) within the first day, with declining levels overtime. Demineralized bone matrix also released detectable amounts of sclerostin into culture medium, while FGF-23 remained close to the detection limit. In vitro expanded isolated bone cells failed to release detectable amounts of sclerostin and FGF-23. CONCLUSION: These results suggest that autografts but also demineralized bone matrix can release signaling molecules that are characteristically produced by osteocytes. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. KEYWORDS: FGF-23; autologous bone; bone grafts; demineralized bone matrix; osteocytes; sclerostin

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery

UniBE Contributor:

Brolese, Eliane Lucia; Buser, Daniel; Kuchler, Ulrike; Schaller, Benoît and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

13 May 2015 11:47

Last Modified:

05 Nov 2015 11:46

Publisher DOI:

10.1111/clr.12432

PubMed ID:

24888411

Uncontrolled Keywords:

autologous bone, bone grafts, demineralized bone matrix, FGF-23, osteocytes, sclerostin

BORIS DOI:

10.7892/boris.68949

URI:

https://boris.unibe.ch/id/eprint/68949

Actions (login required)

Edit item Edit item
Provide Feedback