Thermal processing of bone: in vitro response of mesenchymal cells to bone-conditioned medium

Sawada, Kosaku; Caballé Serrano, Jordi; Schuldt Filho, Günther; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel; Gruber, Reinhard (2015). Thermal processing of bone: in vitro response of mesenchymal cells to bone-conditioned medium. International journal of oral & maxillofacial surgery, 44(8), pp. 1060-1066. Elsevier 10.1016/j.ijom.2015.03.012

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The autoclaving, pasteurization, and freezing of bone grafts to remove bacteria and viruses, and for preservation, respectively, is considered to alter biological properties during graft consolidation. Fresh bone grafts release paracrine-like signals that are considered to support tissue regeneration. However, the impact of the autoclaving, pasteurization, and freezing of bone grafts on paracrine signals remains unknown. Therefore, conditioned medium was prepared from porcine cortical bone chips that had undergone thermal processing. The biological properties of the bone-conditioned medium were assessed by examining the changes in expression of target genes in oral fibroblasts. The data showed that conditioned medium obtained from bone chips that had undergone pasteurization and freezing changed the expression of adrenomedullin, pentraxin 3, BTB/POZ domain-containing protein 11, interleukin 11, NADPH oxidase 4, and proteoglycan 4 by at least five-fold in oral fibroblasts. Bone-conditioned medium obtained from autoclaved bone chips, however, failed to change the expression of the respective genes. Also, when bone-conditioned medium was prepared from fresh bone chips, autoclaving blocked the capacity of bone-conditioned medium to modulate gene expression. These in vitro results suggest that pasteurization and freezing of bone grafts preserve the release of biologically active paracrine signals, but autoclaving does not. Copyright © 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved. KEYWORDS: allogeneic bone; augmentation; autoclaving; autologous bone; bone bank; bone grafts; bone regeneration; bone supernatant; bone-conditioned medium; freezing; pasteurization

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

UniBE Contributor:

Sawada, Kosaku; Caballé Serrano, Jordi; Schuldt Filho, Günther; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0901-5027

Publisher:

Elsevier

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

26 May 2015 16:35

Last Modified:

28 Jan 2016 09:51

Publisher DOI:

10.1016/j.ijom.2015.03.012

PubMed ID:

25868709

Uncontrolled Keywords:

allogeneic bone, augmentation, autoclaving, autologous bone, bone bank, bone grafts, bone regeneration, bone supernatant, bone-conditioned medium, freezing, pasteurization

BORIS DOI:

10.7892/boris.68951

URI:

https://boris.unibe.ch/id/eprint/68951

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