Differentiation between Staphylococcus aureus and coagulase-negative Staphylococcus species by real-time PCR including detection of methicillin resistants in comparison to conventional microbiology testing.

Klaschik, Sven; Lehmann, Lutz Eric; Steinhagen, Folkert; Book, Malte; Molitor, Ernst; Hoeft, Andreas; Stüber, Frank (2015). Differentiation between Staphylococcus aureus and coagulase-negative Staphylococcus species by real-time PCR including detection of methicillin resistants in comparison to conventional microbiology testing. Journal of clinical laboratory analysis, 29(2), pp. 122-128. Wiley 10.1002/jcla.21739

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BACKGROUND Staphylococcus aureus has long been recognized as a major pathogen. Methicillin-resistant strains of S. aureus (MRSA) and methicillin-resistant strains of S. epidermidis (MRSE) are among the most prevalent multiresistant pathogens worldwide, frequently causing nosocomial and community-acquired infections. METHODS In the present pilot study, we tested a polymerase chain reaction (PCR) method to quickly differentiate Staphylococci and identify the mecA gene in a clinical setting. RESULTS Compared to the conventional microbiology testing the real-time PCR assay had a higher detection rate for both S. aureus and coagulase-negative Staphylococci (CoNS; 55 vs. 32 for S. aureus and 63 vs. 24 for CoNS). Hands-on time preparing DNA, carrying out the PCR, and evaluating results was less than 5 h. CONCLUSIONS The assay is largely automated, easy to adapt, and has been shown to be rapid and reliable. Fast detection and differentiation of S. aureus, CoNS, and the mecA gene by means of this real-time PCR protocol may help expedite therapeutic decision-making and enable earlier adequate antibiotic treatment.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy

UniBE Contributor:

Lehmann, Lutz Eric; Book, Malte and Stüber, Frank

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0887-8013

Publisher:

Wiley

Language:

English

Submitter:

Jeannie Wurz

Date Deposited:

23 Jun 2015 08:36

Last Modified:

23 Jan 2018 12:15

Publisher DOI:

10.1002/jcla.21739

PubMed ID:

24796889

Uncontrolled Keywords:

MRSA; PCR; Staphylococcus; bacterial DNA; microbiology

BORIS DOI:

10.7892/boris.69693

URI:

https://boris.unibe.ch/id/eprint/69693

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