The effect of extracellular nucleotides on cytokine production and phagocytosis of macrophages

Hänsli, Christof Karl (2014). The effect of extracellular nucleotides on cytokine production and phagocytosis of macrophages. (Dissertation, Universität Bern, Medizinische Fakultät, Klinik für Viszerale Chirurgie und Medizin, Inselspital Bern)

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Bacterial sepsis is a severe clinical condition, leading to severe sepsis, septic shock, and death. The complex pathophysiology of sepsis is not yet fully understood. Cytokines, released by immune cells such as macrophages, play an important role in the pathophysiology of sepsis. Kupffer cells are the largest population of macrophages in the body. Purinergic signaling, mediated by different nucleosides and nucleotides, and purinergic receptors, has been shown to have various effects on cytokine release, inflammatory processes and the immune system.

In our work with in vitro experiments we studied the effect of extracellular nucleotides on the release of TNFα by primary murine Kupffer cells, and the effect of extracellular nucleotides on the phagocytosis of murine RAW 264.7 and human U-937 cell culture macrophages. Secretion of TNFα was measured using ELISA, phagocytosis of bio particles was measured using a plate reader phagocytosis assay and flow cytometry.

Our experiments show, that extracellular LPS stimulate release of TNFα in murine Kupffer cells and that extracellular nucleotides inhibit this effect in a dose dependent matter. Our other experiments show phagocytosis of fluorescence labeled bio particles by both macrophage cell lines RAW 264.7 and U-937 in a dose dependent manner. The experiments could not show an effect of extracellular nucleotides on phagocytosis of cell culture macrophages.

Item Type:

Thesis (Dissertation)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine

UniBE Contributor:

Beldi, Guido

Subjects:

600 Technology > 610 Medicine & health

Language:

English

Submitter:

Igor Peter Hammer

Date Deposited:

30 Jun 2015 08:25

Last Modified:

28 Nov 2020 02:25

URN:

urn:nbn:ch:bel-bes-1706

Additional Information:

e-Dissertation (edbe)

BORIS DOI:

10.7892/boris.69893

URI:

https://boris.unibe.ch/id/eprint/69893

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