Chondrocytes within osteochondral grafts are more resistant than osteoblasts to tissue culture at 37°C

Bastian, Johannes Dominik; Egli, Rainer J; Ganz, Reinhold; Hofstetter, Willy; Leunig, Michael (2011). Chondrocytes within osteochondral grafts are more resistant than osteoblasts to tissue culture at 37°C. Journal of investigative surgery, 24(1), pp. 28-34. New York, N.Y.: Taylor & Francis 10.3109/08941939.2010.523511

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It is proposed that an ideal osteochondral allograft for cartilage repair consists of a devitalized bone but functional cartilage. The different modes of nutrient supply in vivo for bone (vascular support) and cartilage (diffusion) suggest that a modulation of storage conditions could differentially affect the respective cells, resulting in the proposed allograft. For this purpose, osteochondral tissues from porcine humeral heads were either cultured at 37°C for up to 24 hr or stored at 4°C for 24 hr, the temperature at which osteochondral allografts are routinely stored. Functionality of the cells was assessed by in situ hybridization for transcripts encoding collagen types I and II. At 37°C, a time-dependent significant reduction of the bone surface covered with functional cells was observed with only 5% ± 5% coverage left at 24 hr compared with 41% ± 10% at 0 hr. Similarly, cartilage area containing functional cells was significantly reduced from 84% ± 7% at 0 hr to 70% ± 3% after 24 hr. After 24 hr at 4°C, a significantly reduced amount of functional cells covering bone surfaces was observed (27% ± 5%) but not of cells within the cartilage (79% ± 8%). In the applied experimental setup, bone cells were more affected by tissue culture at 37°C than cartilage cells. Even though chondrocytes appear to be more sensitive to 37°C than to 4°C, the substantially reduced amount of functional bone cells at 37°C warrants further investigation of whether a preincubation of osteochondral allografts at 37°C--prior to regular storage at 4°C--might result in an optimized osteochondral allograft with devitalized bone but viable cartilage.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

UniBE Contributor:

Bastian, Johannes Dominik; Egli, Rainer; Ganz, Reinhold; Hofstetter, Willy and Leunig, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0894-1939

Publisher:

Taylor & Francis

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:21

Last Modified:

10 Jul 2014 16:28

Publisher DOI:

10.3109/08941939.2010.523511

PubMed ID:

21275527

Web of Science ID:

000287741000006

URI:

https://boris.unibe.ch/id/eprint/6995 (FactScience: 212130)

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