Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria.

Pachlopnik, Jana M; Horn, Michael P; Fux, Michaela; Dahinden, Markus; Mandallaz, Michèle; Schneeberger, Dominique; Baldi, Lucia; Vogel, Monique; Stadler, Beda M; Miescher, Sylvia M (2004). Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria. Journal of autoimmunity, 22(1), pp. 43-51. Elsevier 10.1016/j.jaut.2003.09.007

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IgG autoantibodies against the alpha-chain of the high affinity IgE receptor are claimed to play a pathogenetic role in autoimmune urticaria. The best methods for detection of functional autoantibodies are currently the autologous serum skin test and the basophil histamine release assay. A simplified and feasible screening test would facilitate the diagnosis of autoimmune urticaria. Here we offer an explanation for the difficulties in establishing a screening test for autoantibodies directed against the alpha-chain of the high affinity IgE receptor in autoimmune urticaria. Identical autoantibodies in chronic urticaria patients and healthy donors belonging to the natural autoantibody repertoire were found by sequence analysis of anti-alpha-chain autoantibodies isolated by repertoire cloning from antibody libraries. These natural autoantibodies bound to the receptor and triggered histamine release but only if IgE was previously removed from the receptor. Diagnostic assays used for detection of antibodies directed against the IgE receptor may require signal comparison with and without the artificial removal of IgE, immune complexes, and complement in order to avoid false positive or negative results. After IgE removal diagnostic tests will detect natural autoantibodies against the high affinity IgE receptor regardless of whether they are pathogenic or not.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued]
UniBE Contributor:	Fux, Michaela
Subjects:	600 Technology > 610 Medicine & health 500 Science
ISSN:	0896-8411
Publisher:	Elsevier
Language:	English
Submitter:	Dr. Michaela Fux
Date Deposited:	07 Jul 2015 14:37
Last Modified:	05 Dec 2022 14:48
Publisher DOI:	10.1016/j.jaut.2003.09.007
PubMed ID:	14709412
URI:	https://boris.unibe.ch/id/eprint/70130