Detection of one VH antibody sequence in both healthy donors and urticaria patients.

Fux, Michaela; Vogel, Monique; Stadler, Michael B; Stadler, Beda M; Miescher, Sylvia M (2005). Detection of one VH antibody sequence in both healthy donors and urticaria patients. Journal of immunological methods, 307(1-2), pp. 107-117. Elsevier 10.1016/j.jim.2005.09.014

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We have previously isolated anti-FcepsilonRIalpha autoantibodies from phage libraries of healthy donors and urticaria patients. Strikingly, the same antibody, LTMalpha15, was isolated from both libraries. Sequence analysis revealed a germline configuration of the LTMalpha15 variable heavy (V(H)) chain with a slightly mutated variable light (V(L)) chain supporting its classification as a natural autoantibody. Distribution analysis of anti-FcepsilonRIalpha autoantibodies by functional or serological tests delivered conflicting data. For this reason we have developed a new real-time PCR to analyse the distribution of LTMalpha15V(H) in healthy donors and urticaria patients. Our new bioinformatic program permitted the design of a minor groove binder (MGB) TaqMan probe that specifically detected the LTMalpha15V(H). We were able to demonstrate a broad range of rearranged V(H) gene copy number without any correlation to the state of health. Monitoring LTMalpha15V(H) gene copy number in a single donor over a period of 70 days revealed a time-related fluctuation of circulating B cells carrying LTMalpha15V(H). We propose that our real-time PCR may serve as a model for the quantification of natural antibody sequences at a monoclonal level.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)

UniBE Contributor:

Fux, Michaela

Subjects:

600 Technology > 610 Medicine & health
500 Science

ISSN:

0022-1759

Publisher:

Elsevier

Language:

English

Submitter:

Dr. Michaela Fux

Date Deposited:

07 Jul 2015 14:24

Last Modified:

07 Jul 2015 14:24

Publisher DOI:

10.1016/j.jim.2005.09.014

PubMed ID:

16289529

URI:

https://boris.unibe.ch/id/eprint/70131

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