Vogel, Monique; Tschopp, Cornelia; Bobrzynski, Tomasz; Fux, Michaela; Stadler, Michael B; Miescher, Sylvia M; Stadler, Beda M (2004). A highly conserved interspecies V(H) in the human genome. Journal of molecular biology, 341(2), pp. 477-489. Elsevier 10.1016/j.jmb.2004.06.011
Full text not available from this repository. (Request a copy)Idiotype conservation between human and mouse antibodies has been observed in association with various infectious and autoimmune diseases. We have isolated a human anti-idiotypic antibody to a mouse monoclonal anti-IgE antibody (BSW17) suggesting a conserved interspecies idiotype associated with an anti-IgE response. To find the homologue of BSW17 in the human genome we applied the guided selection strategy. Combining V(H) of BSW17 with a human V(L) repertoire resulted in three light chains. The three V(L) chains were then combined with a human V(H) repertoire resulting in three clones specific for human IgE. Surprisingly, one clone, Hu41, had the same epitope specificity and functional in vitro activity as BSW17 and V(H) complementarity-determining regions identical with that of BSW17. Real-time PCR analysis confirmed the presence of the Hu41 V(H) sequence in the human genome. These data document the first example of the isolation of a human antibody where high sequence similarity to the original murine V(H) sequence is associated with common antigen and epitope specificity.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued] |
UniBE Contributor: |
Fux, Michaela |
Subjects: |
600 Technology > 610 Medicine & health 500 Science |
ISSN: |
0022-2836 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Dr. Michaela Fux |
Date Deposited: |
07 Jul 2015 14:34 |
Last Modified: |
05 Dec 2022 14:48 |
Publisher DOI: |
10.1016/j.jmb.2004.06.011 |
PubMed ID: |
15276838 |
URI: |
https://boris.unibe.ch/id/eprint/70132 |
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