A highly conserved interspecies V(H) in the human genome.

Vogel, Monique; Tschopp, Cornelia; Bobrzynski, Tomasz; Fux, Michaela; Stadler, Michael B; Miescher, Sylvia M; Stadler, Beda M (2004). A highly conserved interspecies V(H) in the human genome. Journal of molecular biology, 341(2), pp. 477-489. Elsevier 10.1016/j.jmb.2004.06.011

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Idiotype conservation between human and mouse antibodies has been observed in association with various infectious and autoimmune diseases. We have isolated a human anti-idiotypic antibody to a mouse monoclonal anti-IgE antibody (BSW17) suggesting a conserved interspecies idiotype associated with an anti-IgE response. To find the homologue of BSW17 in the human genome we applied the guided selection strategy. Combining V(H) of BSW17 with a human V(L) repertoire resulted in three light chains. The three V(L) chains were then combined with a human V(H) repertoire resulting in three clones specific for human IgE. Surprisingly, one clone, Hu41, had the same epitope specificity and functional in vitro activity as BSW17 and V(H) complementarity-determining regions identical with that of BSW17. Real-time PCR analysis confirmed the presence of the Hu41 V(H) sequence in the human genome. These data document the first example of the isolation of a human antibody where high sequence similarity to the original murine V(H) sequence is associated with common antigen and epitope specificity.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)

UniBE Contributor:

Fux, Michaela

Subjects:

600 Technology > 610 Medicine & health
500 Science

ISSN:

0022-2836

Publisher:

Elsevier

Language:

English

Submitter:

Dr. Michaela Fux

Date Deposited:

07 Jul 2015 14:34

Last Modified:

07 Jul 2015 14:34

Publisher DOI:

10.1016/j.jmb.2004.06.011

PubMed ID:

15276838

URI:

https://boris.unibe.ch/id/eprint/70132

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