Melanotic tumors of the nervous system are characterized by distinct mutational, chromosomal and epigenomic profiles.

Koelsche, Christian; Hovestadt, Volker; Jones, David T W; Capper, David; Sturm, Dominik; Sahm, Felix; Schrimpf, Daniel; Adeberg, Sebastian; Böhmer, Katja; Hagenlocher, Christian; Mechtersheimer, Gunhild; Kohlhof, Patricia; Mühleisen, Helmut; Beschorner, Rudi; Hartmann, Christian; Braczynski, Anne Kristin; Mittelbronn, Michel; Buslei, Rolf; Becker, Albert; Grote, Alexander; ... (2015). Melanotic tumors of the nervous system are characterized by distinct mutational, chromosomal and epigenomic profiles. Brain pathology, 25(2), pp. 202-208. Blackwell 10.1111/bpa.12228

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Melanotic tumors of the nervous system show overlapping histological characteristics but differ substantially in their biological behavior. In order to achieve a better delineation of such tumors, we performed an in-depth molecular characterization. Eighteen melanocytomas, 12 melanomas, and 14 melanotic and 14 conventional schwannomas (control group) were investigated for methylome patterns (450k array), gene mutations associated with melanotic tumors and copy number variants (CNVs). The methylome fingerprints assigned tumors to entity-specific groups. Methylation groups also showed a substantial overlap with histology-based diagnosis suggesting that they represent true biological entities. On the molecular level, melanotic schwannomas were characterized by a complex karyotype with recurrent monosomy of chromosome 22q and variable whole chromosomal gains and recurrent losses commonly involving chromosomes 1, 17p and 21. Melanocytomas carried GNAQ/11 mutations and presented with CNV involving chromosomes 3 and 6. Melanomas were frequently mutated in the TERT promoter, harbored additional oncogene mutations and showed recurrent chromosomal losses involving chromosomes 9, 10 and 6q, as well as gains of 22q. Together, melanotic nervous system tumors have several distinct mutational and chromosomal alterations and can reliably be distinguished by methylome profiling.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology

UniBE Contributor:

Hewer, Ekkehard

ISSN:

1015-6305

Publisher:

Blackwell

Language:

English

Submitter:

Doris Haefelin

Date Deposited:

13 Jul 2015 10:50

Last Modified:

05 Nov 2015 13:50

Publisher DOI:

10.1111/bpa.12228

PubMed ID:

25399693

Uncontrolled Keywords:

450k; GNA11; GNAQ; TERT promoter; copy number variants; melanocytoma; melanoma; melanotic schwannoma

BORIS DOI:

10.7892/boris.70206

URI:

https://boris.unibe.ch/id/eprint/70206

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