Combined ATRX/IDH1 immunohistochemistry predicts genotype of oligoastrocytomas

Hewer, Ekkehard; Vajtai, Istvan; Dettmer, Matthias; Berezowska, Sabina Anna; Vassella, Erik (2015). Combined ATRX/IDH1 immunohistochemistry predicts genotype of oligoastrocytomas. Histopathology, 68(2), pp. 272-278. Blackwell Scientific Publications 10.1111/his.12743

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AIMS To assess whether in oligoastrocytomas ATRX deficiency - as a surrogate of the alternative lengthening of telomeres (ALT) pathway - has a role in predicting the presence or absence of loss of heterozygosity of 1p and 19q (LOH), the genetic signature of oligodendroglial differentiation and a favourable prognostic marker. METHODS AND RESULTS A series of 54 oligoastrocytomas were investigated by immunohistochemistry as well as microsatellite analysis for LOH 1p19q. Genetic findings were correlated with morphological assessment. CONCLUSIONS ATRX deficiency was mutually exclusive with LOH. Conversely, ATRX-proficient tumours immunoreactive for R132H-mutant isocitrate dehydrogenase 1 (IDH1) showed a high rate (85%) of LOH. A more oligodendroglioma-like morphology was associated with a higher rate of LOH even in the morphologically ambiguous group of oligoastrocytomas. Our findings support the concept that oligoastrocytomas represent a morphological grey zone rather than a group of truly "mixed" or "intermediate" tumours. More precise classification of diffuse gliomas may also improve grading of borderline cases. We propose an immunohistochemical algorithm for classification of morphologically ambiguous diffuse gliomas. This article is protected by copyright. All rights reserved.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology

UniBE Contributor:

Hewer, Ekkehard; Vajtai, Istvan; Dettmer, Matthias; Berezowska, Sabina Anna and Vassella, Erik

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0309-0167

Publisher:

Blackwell Scientific Publications

Language:

English

Submitter:

Doris Haefelin

Date Deposited:

14 Jul 2015 10:08

Last Modified:

27 Apr 2017 16:32

Publisher DOI:

10.1111/his.12743

PubMed ID:

26016385

Uncontrolled Keywords:

Oligoastrocytoma; Alternative lengthening of telomeres; ATRX; IDH1

URI:

https://boris.unibe.ch/id/eprint/70224

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