CD47 protein expression in acute myeloid leukemia: A tissue microarray-based analysis

Galli, Serena; Zlobec, Inti; Schürch, Christian; Perren, Aurel; Ochsenbein, Adrian; Banz Wälti, Yara (2015). CD47 protein expression in acute myeloid leukemia: A tissue microarray-based analysis. Leukemia research, 39(7), pp. 749-756. Elsevier 10.1016/j.leukres.2015.04.007

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Binding of CD47 to signal regulatory protein alpha (SIRPα), an inhibitory receptor, negatively regulates phagocytosis. In acute myeloid leukemia (AML), CD47 is overexpressed on peripheral blasts and leukemia stem cells and inversely correlates with survival. Aim of the study was to investigate the correlation between CD47 protein expression by immunohistochemistry (IHC) in a bone marrow (BM) tissue microarray (TMA) and clinical outcome in AML patients. CD47 staining on BM leukemia blasts was scored semi-quantitatively and correlated with clinical parameters and known prognostic factors in AML. Low (scores 0-2) and high (score 3) CD47 protein expression were observed in 75% and 25% of AML patients. CD47 expression significantly correlated with percentage BM blast infiltration and peripheral blood blasts. Moreover, high CD47 expression was associated with nucleophosmin (NPM1) gene mutations. In contrast, CD47 expression did not significantly correlate with overall or progression free survival or response to therapy. In summary, a BM TMA permits rapid and reproducible semi-quantitative analysis of CD47 protein expression by IHC. While CD47 expression on circulating AML blasts has been shown to be a negative prognostic marker for a very defined population of AML patients with NK AML, CD47 expression on AML BM blasts is not.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Autopsy

UniBE Contributor:

Zlobec, Inti; Schürch, Christian; Perren, Aurel; Ochsenbein, Adrian and Banz Wälti, Yara

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0145-2126

Publisher:

Elsevier

Language:

English

Submitter:

Doris Haefelin

Date Deposited:

14 Jul 2015 11:10

Last Modified:

05 Nov 2015 13:46

Publisher DOI:

10.1016/j.leukres.2015.04.007

PubMed ID:

25943033

Uncontrolled Keywords:

Acute myeloid leukemia; Bone marrow; CD47; Tissue microarray

BORIS DOI:

10.7892/boris.70228

URI:

https://boris.unibe.ch/id/eprint/70228

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