Genetic diversity of bovine Neospora caninum determined by microsatellite markers

Salehi, N; Gottstein, Bruno; Haddadzadeh, H R (2015). Genetic diversity of bovine Neospora caninum determined by microsatellite markers. Parasitology international, 64(5), pp. 357-361. Elsevier 10.1016/j.parint.2015.05.005

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Neospora caninum is one of the most significant parasitic organisms causing bovine abortion worldwide. Despite the economic impact of this infection, relatively little is known about the genetic diversity of this parasite. In this study, using Nc5 and ITS1 nested PCR, N. caninum has been detected in 12 brain samples of aborted fetuses from 298 seropositive dairy cattle collected from four different regions in Tehran, Iran. These specimen (Nc-Iran) were genotyped in multilocus using 9 different microsatellite markers previously described (MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12 and MS21). Microsatellite amplification was completely feasible in 2 samples, semi-completely in 8 samples, and failed in 2 samples. Within the two completely performed allelic profiles of Nc-Iran strains, unique multilocus profiles were obtained for both and novel allelic patterns were found in the MS8 and MS10 microsatellite markers. The Jaccard's similarity index showed significant difference between these two strains and from other standard isolates derived from GenBank such as Nc-Liv, Nc-SweB1, Nc-GER1, KBA1, and KBA2. All samples originating from the same area showed identical allelic numbers and a correlation between the number of repeats and geographic districts was observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

1383-5769

Publisher:

Elsevier

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

27 Jul 2015 14:05

Last Modified:

16 May 2016 02:30

Publisher DOI:

10.1016/j.parint.2015.05.005

PubMed ID:

25988829

Uncontrolled Keywords:

Aborted fetuses; Genotyping; Microsatellite; Neospora caninum; PCR

BORIS DOI:

10.7892/boris.70432

URI:

https://boris.unibe.ch/id/eprint/70432

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