Swart, Estienne Carl; Nowacki, Mariusz (2015). The eukaryotic way to defend and edit genomes by sRNA-targeted DNA deletion. Annals of the New York Academy of Sciences, 1341, pp. 106-114. Blackwell 10.1111/nyas.12636
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While there is currently burgeoning interest in the application of the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) to genome editing, it is perhaps not widely appreciated that this is the second discovery of a small RNA (sRNA)-targeted DNA-deletion system. The first sRNA-targeted DNA-deletion system to be discovered, which we call IES/Ias (internal eliminated sequence/IES-associated genes) to contrast with CRISPR/Cas, is found in ciliates, and, like CRISPR/Cas, is thought to serve as a form of immune defense against invasive DNAs. The manner in which the ciliate IES/Ias system functions is distinct from that of the CRISPR/Cas system in archaea and bacteria, and arose independently through a synthesis of RNA interference-derived and DNA-specific molecular components. Despite the major differences between CRISPR/Cas and IES/Ias, both systems face similar conceptual challenges in targeting invasive DNAs. In this review, we focus on the discovery, effects, function, and evolutionary consequences of the IES/Ias system.
Item Type: |
Journal Article (Review Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate 08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Swart, Estienne Carl, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0077-8923 |
Publisher: |
Blackwell |
Language: |
English |
Submitter: |
Mariusz Nowacki |
Date Deposited: |
18 Aug 2015 16:03 |
Last Modified: |
05 Dec 2022 14:48 |
Publisher DOI: |
10.1111/nyas.12636 |
PubMed ID: |
25581723 |
Uncontrolled Keywords: |
IES/Ias; CRISPR/Cas; sRNA; sRNA-targeted DNA deletion; genome editing; DNA excision |
BORIS DOI: |
10.7892/boris.70921 |
URI: |
https://boris.unibe.ch/id/eprint/70921 |