The eukaryotic way to defend and edit genomes by sRNA-targeted DNA deletion

Swart, Estienne Carl; Nowacki, Mariusz (2015). The eukaryotic way to defend and edit genomes by sRNA-targeted DNA deletion. Annals of the New York Academy of Sciences, 1341, pp. 106-114. Blackwell 10.1111/nyas.12636

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While there is currently burgeoning interest in the application of the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) to genome editing, it is perhaps not widely appreciated that this is the second discovery of a small RNA (sRNA)-targeted DNA-deletion system. The first sRNA-targeted DNA-deletion system to be discovered, which we call IES/Ias (internal eliminated sequence/IES-associated genes) to contrast with CRISPR/Cas, is found in ciliates, and, like CRISPR/Cas, is thought to serve as a form of immune defense against invasive DNAs. The manner in which the ciliate IES/Ias system functions is distinct from that of the CRISPR/Cas system in archaea and bacteria, and arose independently through a synthesis of RNA interference-derived and DNA-specific molecular components. Despite the major differences between CRISPR/Cas and IES/Ias, both systems face similar conceptual challenges in targeting invasive DNAs. In this review, we focus on the discovery, effects, function, and evolutionary consequences of the IES/Ias system.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Swart, Estienne Carl and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0077-8923

Publisher:

Blackwell

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

18 Aug 2015 16:03

Last Modified:

18 Aug 2015 16:03

Publisher DOI:

10.1111/nyas.12636

PubMed ID:

25581723

Uncontrolled Keywords:

IES/Ias; CRISPR/Cas; sRNA; sRNA-targeted DNA deletion; genome editing; DNA excision

BORIS DOI:

10.7892/boris.70921

URI:

https://boris.unibe.ch/id/eprint/70921

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