A 1.8-kb insertion in the 3'-UTR of RXFP2 is associated with polledness in sheep

Wiedemar, Natalie; Drögemüller, Cord (2015). A 1.8-kb insertion in the 3'-UTR of RXFP2 is associated with polledness in sheep. Animal genetics, 46(4), pp. 457-461. Blackwell 10.1111/age.12309

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Sheep breeds show a broad spectrum of different horn phenotypes. In most modern production breeds, sheep are polled (absence of horns), whereas horns occur mainly in indigenous breeds. Previous studies mapped the responsible locus to the region of the RXFP2 gene on ovine chromosome 10. A 4-kb region of the 3'-end of RXFP2 was amplified in horned and polled animals from seven Swiss sheep breeds. Sequence analysis identified a 1833-bp genomic insertion located in the 3'-UTR region of RXFP2 present in polled animals only. An efficient PCR-based genotyping method to determine the polled genotype of individual sheep is presented. Comparative sequence analyses revealed evidence that the polled-associated insertion adds a potential antisense RNA sequence of EEF1A1 to the 3'-end of RXFP2 transcripts.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Wiedemar, Natalie and Drögemüller, Cord

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0268-9146

Publisher:

Blackwell

Language:

English

Submitter:

Cord Drögemüller

Date Deposited:

24 Aug 2015 11:09

Last Modified:

26 Aug 2015 00:40

Publisher DOI:

10.1111/age.12309

PubMed ID:

26103004

Uncontrolled Keywords:

antisense RNA; horn growth; polledness; relaxin/insulin-like family peptide receptor 2; sheep

BORIS DOI:

10.7892/boris.71096

URI:

https://boris.unibe.ch/id/eprint/71096

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