Bone Conditioned Medium: Preparation and Bioassay.

Caballé Serrano, Jordi; Sawada, Kosaku; Schuldt Filho, Günther; Bosshardt, Dieter; Buser, Daniel; Gruber, Reinhard (2015). Bone Conditioned Medium: Preparation and Bioassay. Journal of visualized experiments(101), e52707. MYJoVE Corporation 10.3791/52707

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Autologous bone grafts are widely used in oral and maxillofacial surgery, orthopedics, and traumatology. Autologous bone grafts not only replace missing bone, they also support the complex process of bone regeneration. This favorable behavior of autografts is attributed to the three characteristics: osteoconductivity, osteogenicity, and osteoinductivity. However, there is another aspect: Bone grafts release a myriad of molecules, including growth factors, which can target mesenchymal cells involved in bone regeneration. The paracrine properties of bone grafts can be studied in vitro by the use of bone-conditioned medium (BCM). Here we present a protocol on how to prepare bone-conditioned medium from native pig cortical bone, and bone that underwent thermal processing or demineralization. Cells can be directly exposed to BCM or seeded onto biomaterials, such as collagen membranes, previously soaked with BCM. We give examples for in vitro bioassays with mesenchymal cells on the expression of TGF-β regulated genes. The presented protocols should encourage to further reveal the paracrine effects of bone grafts during bone regeneration and open a path for translational research in the broad field of reconstructive surgery.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

UniBE Contributor:

Caballé Serrano, Jordi; Sawada, Kosaku; Schuldt Filho, Günther; Bosshardt, Dieter; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-087X

Publisher:

MYJoVE Corporation

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

26 Aug 2015 09:23

Last Modified:

08 Aug 2017 02:30

Publisher DOI:

10.3791/52707

PubMed ID:

26273849

Uncontrolled Keywords:

Molecular Biology, Issue 101, Bone Conditioned Medium, BCM, bone autograft, guided bone regeneration, GBR, dental implant, membrane, supernatant, growth factors, contour augmentation, autologous bone

BORIS DOI:

10.7892/boris.71167

URI:

https://boris.unibe.ch/id/eprint/71167

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