Cvikl, Barbara; Lussi, Adrian; Moritz, A; Sawada, Kosaku; Gruber, Reinhard
(2016).
Differential inflammatory response of dental pulp explants and fibroblasts to saliva.
International endodontic journal, 49(7), pp. 655-662.
Wiley-Blackwell
10.1111/iej.12493
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Abstract
AIM:
To investigate the inflammatory response of dental pulp fibroblasts and the respective explants to whole saliva.
METHODOLOGY:
Explants from human and porcine dental pulp tissue and isolated dental pulp fibroblasts were used to investigate the inflammatory response to sterile saliva. Cytokine and chemokine expression was assessed by RT-PCR. Western blot analysis and pharmacologic inhibitors were used to determine the involvement of signalling pathways.
RESULTS:
Dental pulp explants of human and porcine origin exposed to human saliva exhibited no major changes of IL-6 and IL-8 mRNA expression (P > 0.05). In contrast, isolated porcine and human dental pulp fibroblasts, when stimulated with human saliva, exhibited a vastly increased expression of IL-6 and IL-8 mRNA (P < 0.05). In pulp fibroblasts, saliva also increased the expression of other cytokines and chemokines via activation of NFkappaB, ERK and p38 signalling. Notably, a significantly reduced inflammatory response was elicited when pulp fibroblasts were transiently exposed to saliva.
CONCLUSIONS:
Saliva has a potential impact on inflammation of dental pulp fibroblasts in vitro but not when cells are embedded in the intrinsic extracellular matrix of the explant tissue.
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Item Type: |
Journal Article
(Original Article)
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Restorative Dentistry, Research 04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry 04 Faculty of Medicine > School of Dental Medicine 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie 04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research 04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research |
UniBE Contributor: |
Cvikl, Barbara; Lussi, Adrian; Sawada, Kosaku and Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health 000 Computer science, knowledge & systems 300 Social sciences, sociology & anthropology |
ISSN: |
0143-2885 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Caroline Dominique Zürcher
|
Date Deposited: |
24 Aug 2015 15:10 |
Last Modified: |
16 Sep 2019 13:00 |
Publisher DOI: |
10.1111/iej.12493 |
PubMed ID: |
26114806 |
Uncontrolled Keywords: |
cytokines, dental pulp fibroblasts, inflammation, MAPK, NFkappaB, saliva |
BORIS DOI: |
10.7892/boris.71168 |
URI: |
https://boris.unibe.ch/id/eprint/71168 |
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