Differential inflammatory response of dental pulp explants and fibroblasts to saliva

Cvikl, Barbara; Lussi, Adrian; Moritz, A; Sawada, Kosaku; Gruber, Reinhard (2016). Differential inflammatory response of dental pulp explants and fibroblasts to saliva. International endodontic journal, 49(7), pp. 655-662. Wiley-Blackwell 10.1111/iej.12493

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Abstract AIM: To investigate the inflammatory response of dental pulp fibroblasts and the respective explants to whole saliva. METHODOLOGY: Explants from human and porcine dental pulp tissue and isolated dental pulp fibroblasts were used to investigate the inflammatory response to sterile saliva. Cytokine and chemokine expression was assessed by RT-PCR. Western blot analysis and pharmacologic inhibitors were used to determine the involvement of signalling pathways. RESULTS: Dental pulp explants of human and porcine origin exposed to human saliva exhibited no major changes of IL-6 and IL-8 mRNA expression (P > 0.05). In contrast, isolated porcine and human dental pulp fibroblasts, when stimulated with human saliva, exhibited a vastly increased expression of IL-6 and IL-8 mRNA (P < 0.05). In pulp fibroblasts, saliva also increased the expression of other cytokines and chemokines via activation of NFkappaB, ERK and p38 signalling. Notably, a significantly reduced inflammatory response was elicited when pulp fibroblasts were transiently exposed to saliva. CONCLUSIONS: Saliva has a potential impact on inflammation of dental pulp fibroblasts in vitro but not when cells are embedded in the intrinsic extracellular matrix of the explant tissue.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research

UniBE Contributor:

Cvikl, Barbara; Lussi, Adrian; Sawada, Kosaku and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health
000 Computer science, knowledge & systems
300 Social sciences, sociology & anthropology

ISSN:

0143-2885

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

24 Aug 2015 15:10

Last Modified:

16 Sep 2019 13:00

Publisher DOI:

10.1111/iej.12493

PubMed ID:

26114806

Uncontrolled Keywords:

cytokines, dental pulp fibroblasts, inflammation, MAPK, NFkappaB, saliva

BORIS DOI:

10.7892/boris.71168

URI:

https://boris.unibe.ch/id/eprint/71168

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