Simultaneous analysis of nuclear and mitochondrial DNA, mRNA and miRNA from backspatter from inside parts of firearms generated by shots at "triple contrast" doped ballistic models

Grabmüller, Melanie; Schyma, Christian; Euteneuer, Jan; Madea, Burkhard; Courts, Cornelius (2015). Simultaneous analysis of nuclear and mitochondrial DNA, mRNA and miRNA from backspatter from inside parts of firearms generated by shots at "triple contrast" doped ballistic models. Forensic science, medicine, and pathology, 11(3), pp. 365-375. Springer 10.1007/s12024-015-9695-3

[img] Text
art%3A10.1007%2Fs12024-015-9695-3.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (709kB) | Request a copy

When a firearm projectile hits a biological target a spray of biological material (e.g., blood and tissue fragments) can be propelled from the entrance wound back towards the firearm. This phenomenon has become known as "backspatter" and if caused by contact shots or shots from short distances traces of backspatter may reach, consolidate on, and be recovered from, the inside surfaces of the firearm. Thus, a comprehensive investigation of firearm-related crimes must not only comprise of wound ballistic assessment but also backspatter analysis, and may even take into account potential correlations between these emergences. The aim of the present study was to evaluate and expand the applicability of the "triple contrast" method by probing its compatibility with forensic analysis of nuclear and mitochondrial DNA and the simultaneous investigation of co-extracted mRNA and miRNA from backspatter collected from internal components of different types of firearms after experimental shootings. We demonstrate that "triple contrast" stained biological samples collected from the inside surfaces of firearms are amenable to forensic co-analysis of DNA and RNA and permit sequence analysis of the entire mtDNA displacement-loop, even for "low template" DNA amounts that preclude standard short tandem repeat DNA analysis. Our findings underscore the "triple contrast" method's usefulness as a research tool in experimental forensic ballistics.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Medicine

UniBE Contributor:

Schyma, Christian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1556-2891

Publisher:

Springer

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

02 Sep 2015 09:19

Last Modified:

07 Aug 2017 13:20

Publisher DOI:

10.1007/s12024-015-9695-3

PubMed ID:

26210238

BORIS DOI:

10.7892/boris.71374

URI:

https://boris.unibe.ch/id/eprint/71374

Actions (login required)

Edit item Edit item
Provide Feedback