Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility

Talman, Arthur M; Prieto, Judith H; Marques, Sara; Ubaida-Mohien, Ceereena; Lawniczak, Mara; Wass, Mark N; Xu, Tao; Frank, Roland; Ecker, Andrea; Limenitakis Stanway, Rebecca; Krishna, Sanjeev; Sternberg, Michael J E; Christophides, Georges K; Graham, David R; Dinglasan, Rhoel R; Yates, John R; Sinden, Robert E (2014). Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility. Malaria journal, 13(315), p. 315. BioMed Central 10.1186/1475-2875-13-315

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BACKGROUND Gametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells and are proven targets of transmission blocking immunity, little is known about their molecular organization. For example, the pathway of energy metabolism that power motility, a feature that facilitates gamete encounter and fertilization, is unknown. METHODS Plasmodium berghei microgametes were purified and analysed by whole-cell proteomic analysis for the first time. Data are available via ProteomeXchange with identifier PXD001163. RESULTS 615 proteins were recovered, they included all male gamete proteins described thus far. Amongst them were the 11 enzymes of the glycolytic pathway. The hexose transporter was localized to the gamete plasma membrane and it was shown that microgamete motility can be suppressed effectively by inhibitors of this transporter and of the glycolytic pathway. CONCLUSIONS This study describes the first whole-cell proteomic analysis of the malaria male gamete. It identifies glycolysis as the likely exclusive source of energy for flagellar beat, and provides new insights in original features of Plasmodium flagellar organization.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria

UniBE Contributor:

Limenitakis Stanway, Rebecca

Subjects:

500 Science > 570 Life sciences; biology
500 Science

ISSN:

1475-2875

Publisher:

BioMed Central

Language:

English

Submitter:

Volker Heussler

Date Deposited:

10 Sep 2015 15:05

Last Modified:

26 Jun 2016 02:06

Publisher DOI:

10.1186/1475-2875-13-315

PubMed ID:

25124718

BORIS DOI:

10.7892/boris.71394

URI:

https://boris.unibe.ch/id/eprint/71394

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