Homing of placenta-derived mesenchymal stem cells after perinatal intracerebral transplantation in a rat model

Schoeberlein, Andreina; Mueller, Martin; Reinhart, Ursula; Sager, Ruth; Messerli, Marianne; Surbek, Daniel V (2011). Homing of placenta-derived mesenchymal stem cells after perinatal intracerebral transplantation in a rat model. American journal of obstetrics and gynecology, 205(3), 277.e1-6. Orlando, Fla.: Elsevier 10.1016/j.ajog.2011.06.044

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OBJECTIVE: The aim of this study is to assess early homing of placenta-derived stem cells after perinatal intracerebral transplantation in rats. STUDY DESIGN: Neonatal Wistar rats (2-4 days old) were anesthetized, and 250,000 human placenta-derived mesenchymal stem cells (MSC) injected into the lateral ventricle or the paraventricular white matter using a stereotactic frame. Donor MSC were detected by immunohistochemistry using an antihuman HLA-ABC antibody. RESULTS: In all, 84% of the animals survived the transplantation. Donor cells were detected in the brain ventricle 1-2 hours posttransplantation. After 4 hours, donor cells migrated throughout the ventricular system. At 1-4 weeks after transplantation, some cells had migrated into the periventricular white matter. CONCLUSION: Human placenta-derived MSC were successfully transplanted into the lateral ventricles of neonatal rats. Donor cells survived, homed, and migrated in the recipient brains. Proliferation and differentiation analysis and functional tests will assess the therapeutic effects of stem cell transplantation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

UniBE Contributor:

Schoeberlein, Andreina; Müller, Andrea Martin and Surbek, Daniel

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0002-9378

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:21

Last Modified:

04 Nov 2019 13:52

Publisher DOI:

10.1016/j.ajog.2011.06.044

PubMed ID:

22071064

Web of Science ID:

000294330000057

BORIS DOI:

10.7892/boris.7143

URI:

https://boris.unibe.ch/id/eprint/7143 (FactScience: 212319)

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