Uruburuella testudinis sp. nov., isolated from tortoise (Testudo)

Kuhnert, Peter; Thomann, Andreas; Brodard, Isabelle; Haefeli, Willi; Korczak, Bozena (2015). Uruburuella testudinis sp. nov., isolated from tortoise (Testudo). International journal of systematic and evolutionary microbiology, 65(Pt 4), pp. 1251-1255. Society for General Microbiology SGM 10.1099/ijs.0.000089

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A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene similarities between strains of 99.5-100%, and of up to 98.2% with U. suis . DNA-DNA hybridization indicated the organisms represented a novel species with only 40% DNA-DNA similarity with U. suis . Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae . The major fatty acids were C(16 : 0) and summed feature C(16 : 1)ω7c/iso-C(15 : 0) 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_OD624(T) ( = DSM 26510(T) = CCUG 63373(T)).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter; Thomann, Andreas; Brodard, Isabelle and Korczak, Bozena

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

17 Sep 2015 11:05

Last Modified:

17 Sep 2015 11:05

Publisher DOI:

10.1099/ijs.0.000089

PubMed ID:

25634951

BORIS DOI:

10.7892/boris.71678

URI:

https://boris.unibe.ch/id/eprint/71678

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