Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

Wass, M N; Limenitakis Stanway, Rebecca; Blagborough, A M; Lal, K; Prieto, J H; Raine, D; Sternberg, M J E; Talman, A M; Tomley, F; Yates, J; Sinden, R E (2012). Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls. Parasitology, 139(9), pp. 1131-1145. Cambridge University Press 10.1017/S0031182012000133

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Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria

UniBE Contributor:

Limenitakis Stanway, Rebecca

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Volker Heussler

Date Deposited:

21 Sep 2015 14:38

Last Modified:

27 Apr 2018 08:38

Publisher DOI:

10.1017/S0031182012000133

PubMed ID:

22336136

BORIS DOI:

10.7892/boris.71855

URI:

https://boris.unibe.ch/id/eprint/71855

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