Cold Microwave-Enabled Protein Detection and Quantification.

Grützner, Niels; Heilmann, Romy M; Smith, Aaron G; Johnson, Carol B; Vitha, Stanislav; Steiner, Jörg M; Holzenburg, Andreas K (2015). Cold Microwave-Enabled Protein Detection and Quantification. In: Kurien, Bijt T.; Scofield, Hal R. (eds.) Detection of Blotted Proteins. Methods and Protocols. Methods in Molecular Biology: Vol. 1314 (pp. 207-217). New York: Humana Press 10.1007/978-1-4939-2718-0_23

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Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio is highly beneficial in many research areas. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, Western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 24-45 min.

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