Cold Microwave-Enabled Protein Detection and Quantification.

Grützner, Niels; Heilmann, Romy M; Smith, Aaron G; Johnson, Carol B; Vitha, Stanislav; Steiner, Jörg M; Holzenburg, Andreas K (2015). Cold Microwave-Enabled Protein Detection and Quantification. In: Kurien, Bijt T.; Scofield, Hal R. (eds.) Detection of Blotted Proteins. Methods and Protocols. Methods in Molecular Biology: Vol. 1314 (pp. 207-217). New York: Humana Press 10.1007/978-1-4939-2718-0_23

[img] Text
Grützner 2015 Cold Microwave-enabled protein detection and quantification.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (493kB) | Request a copy

Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio is highly beneficial in many research areas. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, Western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 24-45 min.

Item Type:

Book Section (Book Chapter)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Grützner, Niels

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1064-3745

ISBN:

978-1-4939-2717-3

Series:

Methods in Molecular Biology

Publisher:

Humana Press

Language:

English

Submitter:

Daniela Fasel

Date Deposited:

23 Oct 2015 16:05

Last Modified:

26 Feb 2016 14:58

Publisher DOI:

10.1007/978-1-4939-2718-0_23

PubMed ID:

26139270

BORIS DOI:

10.7892/boris.72206

URI:

https://boris.unibe.ch/id/eprint/72206

Actions (login required)

Edit item Edit item
Provide Feedback